Escola de Química-EQ/UFRJ, CT, Bloco E, Rio de Janeiro, 21945-900, RJ, Brazil.
Appl Biochem Biotechnol. 2010 Apr;160(8):2498-508. doi: 10.1007/s12010-009-8756-z. Epub 2009 Aug 24.
This work reports the application of a lipase in the 2-ethylhexyl palmitate esterification in a solvent-free system with an immobilized lipase (Lipozyme RM IM). A sequential strategy was used applying two experimental designs to optimize the 2-ethylhexyl palmitate production. An empirical model was then built so as to assess the effects of process variables on the reaction conversion. Afterwards, the operating conditions that optimized 2-ethylhexyl palmitate production were established as being acid/alcohol molar ratio 1:3, temperature of 70 degrees C, stirring rate of 150 rpm, 10 wt.% of enzyme, leading to a reaction conversion as high as 95%. From this point, a kinetic study was carried out evaluating the effect of acid:alcohol molar ratio, the enzyme concentration and the temperature on product conversion. The results obtained in this step permit to verify that an excess of alcohol (acid to alcohol molar ratio of 1:6), relatively low enzyme concentration (10 wt.%) and temperature of 70 degrees C, led to conversions next to 100%.
本工作报道了脂肪酶在无溶剂体系中用于 2-乙基己基棕榈酸酯酯化的应用，使用固定化脂肪酶（Lipozyme RM IM）。采用顺序策略应用两种实验设计来优化 2-乙基己基棕榈酸酯的生产。然后建立经验模型，以评估工艺变量对反应转化率的影响。之后，确定了优化 2-乙基己基棕榈酸酯生产的操作条件为酸/醇摩尔比 1:3、温度 70°C、搅拌速度 150rpm、酶用量 10wt%，反应转化率高达 95%。从这一点出发，进行了动力学研究，评估了酸：醇摩尔比、酶浓度和温度对产物转化率的影响。这一步的结果验证了醇的过量（酸：醇摩尔比为 1:6）、相对较低的酶浓度（10wt%）和 70°C 的温度导致转化率接近 100%。
