Sjövall M, Ekblad E, Lundell L, Sundler F
Department of Surgery, Sahlgren's Hospital, University of Gothenburg, Sweden.
Regul Pept. 1990 Mar 27;28(1):47-55. doi: 10.1016/0167-0115(90)90063-3.
By using immunocytochemical techniques, we have studied the distribution of gastrin releasing peptide (GRP)-containing neurons as well as the spatial relationship between these neurons and the endocrine cells in the human stomach and duodenum. Moderate numbers of immunoreactive fibers were distributed in the smooth muscle and submucosa of the stomach; they were more rare in the duodenal wall. Numerous GRP-containing nerve fibers were found in the oxyntic mucosa, the antral mucosa harboured only few GRP immunoreactive nerve fibers. The mucosa of the proximal duodenum was found to be virtually devoid of such fibers. Only occasionally did we observe signs of a direct contact between GRP-containing nerve fibers and gastrin and somatostatin cells in the antral mucosa. In the oxyntic mucosa GRP-containing nerve fibers sometimes seemed to contact endocrine cells, including somatostatin cells as well as individual parietal cells. In conclusion, although GRP-containing nerve fibers were quite numerous in the wall of the human upper gastro-intestinal (GI)-tract, we observed a lack of intimate spatial relationship between these fibers and endocrine cells in the antral mucosa, suggesting additive mechanisms to a direct innervation of gastrin cells and somatostatin cells by GRP nerve fibers explaining the physiological effects on hormonal release.
通过使用免疫细胞化学技术，我们研究了含胃泌素释放肽（GRP）的神经元的分布，以及这些神经元与人类胃和十二指肠内分泌细胞之间的空间关系。中等数量的免疫反应性纤维分布在胃的平滑肌和黏膜下层；它们在十二指肠壁中较为少见。在胃底黏膜中发现了大量含GRP的神经纤维，而胃窦黏膜中仅含有少量GRP免疫反应性神经纤维。发现十二指肠近端的黏膜几乎没有此类纤维。我们仅偶尔观察到含GRP的神经纤维与胃窦黏膜中的胃泌素和生长抑素细胞之间有直接接触的迹象。在胃底黏膜中，含GRP的神经纤维有时似乎与内分泌细胞接触，包括生长抑素细胞以及单个壁细胞。总之，尽管在人类上消化道（GI）壁中含GRP的神经纤维数量相当多，但我们观察到这些纤维与胃窦黏膜中的内分泌细胞之间缺乏紧密的空间关系，这表明除了GRP神经纤维对胃泌素细胞和生长抑素细胞的直接神经支配外，还存在其他机制来解释对激素释放的生理影响。
