Andersson K, Chen D, Håkanson R, Mattsson H, Sundler F
Gastrointestinal Pharmacology, Preclinical Research and Development, Astra Hässle AB, Mölndal, Sweden.
Cell Tissue Res. 1992 Oct;270(1):7-13. doi: 10.1007/BF00381874.
In the rat, gastric histamine is stored predominantly in the enterochromaffin-like (ECL) cells, which are located basally in the oxyntic mucosa. The functional significance of histamine in the ECL cells is a matter of speculation. In this study the effect of depletion of histamine on the properties and ultrastructure of the ECL cells was examined. Histamine synthesis was inhibited with alpha-fluoromethylhistidine (3 mg.kg-1.h-1) given via osmotic minipumps over a period of 24 h. The treatment reduced the histidine decarboxylase activity (approximately 20% remaining) and histamine concentration (less than 20% remaining) in the oxyntic mucosa, as well as the intensity of histamine- and chromogranin A-immunostaining in the ECL cells, compared to control rats. The cytoplasmic (secretory) granules/vesicles were greatly reduced in number and size following alpha-fluoromethylhistidine administration. The histamine immunostaining of the mast cells, which occurs at the mucosal surface and in the submucosa, appeared unaffected. We conclude that ECL cell histamine accounts for at least 80% of the total oxyntic mucosal histamine in the rat and that it represents a more mobile pool than mast cell histamine. The reduction in the number and size of the ECL cell granules/vesicles following histamine depletion is in accord with the idea that they represent the storage site for histamine.
在大鼠中，胃组胺主要储存于肠嗜铬样（ECL）细胞中，这些细胞位于胃底黏膜的基部。组胺在ECL细胞中的功能意义尚属推测。在本研究中，检测了组胺耗竭对ECL细胞特性和超微结构的影响。通过渗透微型泵在24小时内给予α-氟甲基组氨酸（3毫克·千克-1·小时-1）以抑制组胺合成。与对照大鼠相比，该处理降低了胃底黏膜中的组氨酸脱羧酶活性（约20%残留）和组胺浓度（小于20%残留），以及ECL细胞中组胺和嗜铬粒蛋白A免疫染色的强度。给予α-氟甲基组氨酸后，细胞质（分泌）颗粒/囊泡的数量和大小大大减少。发生在黏膜表面和黏膜下层的肥大细胞的组胺免疫染色似乎未受影响。我们得出结论，ECL细胞组胺占大鼠胃底黏膜总组胺的至少80%，并且它比肥大细胞组胺代表一个更易移动的储存库。组胺耗竭后ECL细胞颗粒/囊泡数量和大小的减少与它们代表组胺储存部位的观点一致。
