Centro Nacional de Biotecnología del CSIC, Campus de la UAM, Darwin 3, Cantoblanco, 28049 Madrid, Spain.
Appl Microbiol Biotechnol. 2010 Feb;85(6):1809-19. doi: 10.1007/s00253-009-2195-1. Epub 2009 Aug 26.
From cell-free extracts of Streptomyces RGU5.3, a tailoring activity of pimaricin, leading to the biosynthesis of its natural carboxamide derivative AB-400, was recently identified. The two polyene macrolides, pimaricin and AB-400, were produced in almost equal quantities and can be detected in the fermentation broth of the producer strain. This report concerns the isolation and partial characterization of the gene, polyene carboxamide synthase (pcsB), responsible for the bioconversion. The gene encoded an asparagine synthase-like protein, belonging to the type II glutamine amidotransferase family, and was named pcsB. The fermentation broth of a recombinant strain carrying the engineered pcsB gene under the control of the inducible tipA promoter within an integrative vector produces the carboxamide AB-400 as the main polyene macrolide.
从链霉菌 RGU5.3 的无细胞提取物中，最近发现了一种对匹马霉素进行修饰的活性，导致其天然羧酰胺衍生物 AB-400 的生物合成。这两种聚烯大环内酯，匹马霉素和 AB-400，几乎以等量产生，并可以在产生菌的发酵液中检测到。本报告涉及负责生物转化的基因，聚烯羧酰胺合酶（pcsB）的分离和部分特性。该基因编码一种天冬酰胺合酶样蛋白，属于 II 型谷氨酰胺酰胺转移酶家族，被命名为 pcsB。在整合载体中，受诱导型 tipA 启动子控制的工程化 pcsB 基因的重组菌株的发酵液中产生羧酰胺 AB-400 作为主要的聚烯大环内酯。
