Wirsel Stefan G R
Lehrstuhl Phytopathologie, Fachbereich Biologie, Universität Konstanz, Universitätstrasse 10, Konstanz, Germany.
FEMS Microbiol Ecol. 2004 May 1;48(2):129-38. doi: 10.1016/j.femsec.2004.01.006.
A molecular approach was applied to investigate the colonisation of arbuscular mycorrhizal fungi (AMF) on the wetland grass Phragmites australis. A PCR assay targeting the traditional families of the Glomeromycota yielded products that were used to construct libraries of 18S rDNA. Five hundred and forty six clones were typed by restriction analysis and 76 representatives were sequenced. The majority corresponded to a wide range of taxa within Glomus group A, a few belonged to the "Diversisporaceae" and none to the genera Scutellospora or Acaulospora. Among these sequences, some were very similar to those reported earlier, e.g. Glomus mosseae and G. fasciculatum, other pointed to various new taxa. Although this wetland habitat harboured just one single plant species, phylogenetic analysis exhibited 21 AMF phylotypes, which is in the same range as reported for other natural ecosystems composed of more diverse host communities. Diversity indices supported the perception that the AMF mycoflora associated with this natural grass "monoculture" is not depauperate as it had been described for grasses of crop monocultures. Soil conditions determined the mycorrhizal state of the host, since AMF were not detected at the lakeward front of the reed belt, which is permanently waterlogged.
采用分子方法研究丛枝菌根真菌（AMF）在湿地植物芦苇上的定殖情况。针对球囊菌门传统科属的PCR分析产生了用于构建18S rDNA文库的产物。通过限制性分析对546个克隆进行分型，并对76个代表克隆进行测序。大多数克隆对应于球囊霉属A组内的广泛分类群，少数属于“多样孢科”，没有属于盾巨孢囊霉属或无梗囊霉属的。在这些序列中，一些与先前报道的序列非常相似，例如摩西球囊霉和聚丛球囊霉，其他序列则指向各种新的分类群。尽管这个湿地栖息地只有一种植物，但系统发育分析显示有21种AMF系统型，这与报道的由更多样化宿主群落组成的其他自然生态系统中的范围相同。多样性指数支持了这样一种观点，即与这种天然草“单一栽培”相关的AMF真菌区系并不像作物单一栽培的草那样贫瘠。土壤条件决定了宿主的菌根状态，因为在芦苇带向湖一侧（永久积水）未检测到AMF。
