Huang Zhengyu, Bonsall Robert F, Mavrodi Dmitri V, Weller David M, Thomashow Linda S
USDA Agricultural Research Service, Root Disease and Biological Control Research Unit, Washington State University, Pullman, WA 99164-6430, USA.
FEMS Microbiol Ecol. 2004 Aug 1;49(2):243-51. doi: 10.1016/j.femsec.2004.03.010.
A seven-gene operon for the synthesis of phenazine-1-carboxylic acid was introduced into Pseudomonas fluorescens Q8r1-96, an aggressive root colonizer that produces 2,4-diacetylphloroglucinol and consistently suppresses take-all of wheat. The recombinant strains produced both antifungal metabolites and maintained population sizes comparable to those of Q8r1-96 over a seven-week period in the rhizosphere of wheat. The strains were no more suppressive of take-all or Pythium root rot than was Q8r1-96, but suppressed Rhizoctonia root rot at a dose of only 10(2) CFU per seed, one to two orders of magnitude lower than the dose of Q8r1-96 required for comparable disease control.
将一个用于合成吩嗪 -1- 羧酸的七基因操纵子导入荧光假单胞菌Q8r1 - 96中，该菌是一种具有侵略性的根部定殖菌，能产生2,4 - 二乙酰基间苯三酚，并持续抑制小麦全蚀病。重组菌株在小麦根际七周的时间内既产生抗真菌代谢产物，又能维持与Q8r1 - 96相当的种群数量。这些菌株对小麦全蚀病或腐霉菌根腐病的抑制作用并不比Q8r1 - 96更强，但在每粒种子仅10(2) CFU的剂量下就能抑制立枯丝核菌根腐病，这一比Q8r1 - 96控制类似病害所需剂量低一到两个数量级。
