YB-10对小麦全蚀病的生物防治及生长促进作用
Biological Control of Take-All and Growth Promotion in Wheat by YB-10.
作者信息
Xu Wen, Xu Lingling, Deng Xiaoxu, Goodwin Paul H, Xia Mingcong, Zhang Jie, Wang Qi, Sun Runhong, Pan Yamei, Wu Chao, Yang Lirong
机构信息
Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Henan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
出版信息
Pathogens. 2021 Jul 17;10(7):903. doi: 10.3390/pathogens10070903.
Wheat is a worldwide staple food crop, and take-all caused by var. can lead to a tremendous decrease in wheat yield and quality. In this study, strain YB-10 was isolated from wheat rhizospheric soil and identified as by morphology and 16S rRNA gene sequencing. YB-10 had extracellular protease and cellulase activities and strongly inhibited the mycelium growth of var. in dual cultures. Up to 87% efficacy of YB-10 in controlling the take-all of seedlings was observed in pot experiments when wheat seed was coated with the bacterium. YB-10 was also positive for indole acetic acid (IAA) and siderophore production, and coating wheat seed with the bacterium significantly promoted the growth of seedlings at 10 and 10 CFU/mL. Furthermore, treatment with YB-10 increased activities of the wheat defense-related enzymes POD, SOD, CAT, PAL and PPO in seedlings, indicating induced resistance against pathogens. Overall, YB-10 is a promising new seed-coating agent to both promote wheat growth and suppress take-all.
小麦是一种全球范围内的主食作物，由禾顶囊壳小麦变种引起的全蚀病会导致小麦产量和品质大幅下降。在本研究中，从小麦根际土壤中分离出菌株YB - 10，并通过形态学和16S rRNA基因测序将其鉴定为[具体菌种]。YB - 10具有胞外蛋白酶和纤维素酶活性，在双培养中强烈抑制禾顶囊壳小麦变种的菌丝生长。在盆栽试验中，当用该细菌包衣小麦种子时，观察到YB - 10对控制幼苗全蚀病的防治效果高达87%。YB - 10还能产生吲哚乙酸（IAA）和铁载体，用该细菌包衣小麦种子在10⁶和10⁷CFU/mL时显著促进了幼苗生长。此外，用YB - 10处理增加了小麦幼苗中与防御相关的酶POD、SOD、CAT、PAL和PPO的活性，表明诱导了对病原体的抗性。总体而言，YB - 10是一种有前景的新型种子包衣剂，既能促进小麦生长又能抑制全蚀病。
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