Gunawan S, Walton N Y, Treiman D M
Neurology, Research Service, Veterans Administration Medical Center, Los Angeles, CA 90073.
J Chromatogr. 1990 Mar 9;503(1):177-87. doi: 10.1016/s0021-9673(01)81499-0.
We describe here a simple, sensitive, selective and reproducible assay method for quantitative determination of aspartate, glutamate, serine, glutamine, glycine and gamma-aminobutyric acid in rat brain using reversed-phase high-performance liquid chromatography. The method is based upon formation of phenylthiocarbamyl derivatives of the amino acids. Good resolution of the six amino acids and the internal standard norvaline is achieved within 40 min. Other amino acids which have been reported to be present in rat brain do not interfere with the analysis. Standard curves for each of the amino acids exhibited good linearity (r greater than 0.9993) over the range 0.5-20 nmol. The coefficient of variation for the intra-day and inter-day determinations ranged from 0.4% at the highest to 11% at the lowest concentration limit. Storage of whole brains at -0 degrees C for up to 8 weeks did not affect mean concentrations of the six amino acids.
我们在此描述一种简单、灵敏、选择性好且可重复的检测方法，用于使用反相高效液相色谱法定量测定大鼠脑中的天冬氨酸、谷氨酸、丝氨酸、谷氨酰胺、甘氨酸和γ-氨基丁酸。该方法基于氨基酸的苯硫代甲酰胺衍生物的形成。在40分钟内可实现六种氨基酸和内标正缬氨酸的良好分离。据报道存在于大鼠脑中的其他氨基酸不会干扰分析。每种氨基酸的标准曲线在0.5 - 20 nmol范围内均表现出良好的线性（r大于0.9993）。日内和日间测定的变异系数在最高浓度时为0.4%，在最低浓度限时为11%。将全脑在 - 0℃下储存长达8周不会影响六种氨基酸的平均浓度。
