Peinado J M, McManus K T, Myers R D
J Neurosci Methods. 1986 Nov;18(3):269-76. doi: 10.1016/0165-0270(86)90013-0.
A simplified method is described for the isocratic analysis of endogenous amino acid neurotransmitters contained in brain perfusates by high performance liquid chromatography (HPLC) with electrochemical detection (EC). Pre-column o-phthalaldehyde (OPA) tert-butylthiol derivatives of the amino acids were injected into a C18 3 microns column. After linear concentration curves for standard solutions were obtained, the content of 6 amino acid neurotransmitters was analyzed in push-pull perfusates obtained from the hypothalamus and cerebral cortex of the unrestrained rat. Each analysis which included the simultaneous quantification of aspartic acid, glutamic acid, glutamine, glycine, taurine and gamma-aminobutyric acid (GABA), was completed in less than 15 min. The sensitivity of the assay ranged from 1.0 to 5.0 pmol of each amino acid contained within a 20 microliters aliquot of each perfusion sample.
描述了一种简化方法，用于通过高效液相色谱（HPLC）结合电化学检测（EC）对等分脑灌注液中所含内源性氨基酸神经递质进行等度分析。将氨基酸的柱前邻苯二甲醛（OPA）叔丁硫醇衍生物注入3微米的C18柱中。在获得标准溶液的线性浓度曲线后，分析了从无束缚大鼠的下丘脑和大脑皮层获得的推挽灌注液中6种氨基酸神经递质的含量。每次分析包括同时定量天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸、牛磺酸和γ-氨基丁酸（GABA），均在不到15分钟内完成。该测定法的灵敏度为每个灌注样品20微升等分试样中所含每种氨基酸1.0至5.0皮摩尔。
