Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
J Sep Sci. 2009 Dec;32(23-24):4254-60. doi: 10.1002/jssc.200900415.
This work demonstrates the potential of HPCE in the analysis of antibiotics in a complex matrix such as feedstuffs. Using 20 mM citric acid-40 mM Na(2)HPO(4) buffer (pH 2.65), the five antibiotics, tetracycline, oxytetracycline, doxycycline, tilmicosin, and tylosin were successfully separated at 30 kV in a 64.5 cm x 75 microm id capillary. Good repeatability, stability, and reliability of the method were supported by <10% CV with mean recoveries of >70%, and the limit of detection of the five analytes was 0.5-1 mg/kg. It was for the first time that a capillary electrophoretic method was employed to simultaneously detect five tetracycline and macrolide antibiotics in animal feeds.
本工作展示了 HPCE 在分析饲料等复杂基质中的抗生素的潜力。使用 20 mM 柠檬酸-40 mM Na2HPO4 缓冲液（pH 2.65），在 30 kV 下，成功分离了五种抗生素，即四环素、土霉素、强力霉素、替米考星和泰乐菌素，在 64.5 cm x 75 微米内径的毛细管中。该方法具有良好的重复性、稳定性和可靠性，其平均值回收率>70%，CV<10%，五种分析物的检测限为 0.5-1 mg/kg。这是首次采用毛细管电泳法同时检测动物饲料中的五种四环素和大环内酯类抗生素。
