Rakovskaia I V, Gorina L G, Barkhatova O I, Balabanov D N, Goncharova S A, Gamova N A, Levina G A
Zh Mikrobiol Epidemiol Immunobiol. 2009 Jul-Aug(4):81-5.
To study the possibility of existence of antigenemia during urogenital mycoplasmal infections by detection the antigens of agents in blood and viscera of infected animals.
Rabbits and mice were intraperitoneally inoculated with Mycoplasma hominis and Ureaplasma urealyticum, their antigens and DNAs. Samples of blood and visceral organs were studied by several methods: cultural with use of standard media, PCR, RT-PCR, indirect hemagglutination test, and immunofluorescence assay for detection of antibodies.
Bacteremia with M. hominis develops during 2 months after inoculation in rabbits and 3 weeks after inoculation in mice. Antigens of M. hominis and U. urealyticum were detected in serum and visceral organs significantly frequently than live cells and DNAs. Prolonged preservation of the antigens in animals' blood and viscera after intraperitoneal administration of "pure" antigens points to the presence of true mycoplasmal antigenemia. Forms of existence of antigens in organism are different-they can represent corpuscular antigens as well as soluble molecular compounds circulating in blood both in free state and in structure of immune complexes. Antigens as well as live cells are preserved in all studied organs.
Inoculation of rabbits and mice with M. hominis or U. urealyticum resulted in development of generalized infection with persistence of the agent in all studied organs during initial phase of infection and predominant persistence in organs of immunogenesis during later phases.
通过检测感染动物血液和内脏中病原体的抗原，研究泌尿生殖系统支原体感染期间抗原血症存在的可能性。
给家兔和小鼠腹腔接种人型支原体、解脲脲原体及其抗原和DNA。采用多种方法研究血液和内脏器官样本：使用标准培养基培养、聚合酶链反应（PCR）、逆转录聚合酶链反应（RT-PCR）、间接血凝试验以及用于检测抗体的免疫荧光测定法。
接种后，家兔在2个月内、小鼠在3周内出现人型支原体菌血症。在血清和内脏器官中检测到人型支原体和解脲脲原体抗原的频率明显高于活细胞和DNA。腹腔注射“纯”抗原后，动物血液和内脏中的抗原长时间存在，这表明存在真正的支原体抗原血症。抗原在机体中的存在形式不同——它们可以是颗粒性抗原，也可以是游离状态或免疫复合物结构中循环于血液中的可溶性分子化合物。抗原和活细胞在所有研究的器官中均有留存。
给家兔和小鼠接种人型支原体或解脲脲原体导致全身感染，在感染初期病原体在所有研究器官中持续存在，在后期主要在免疫发生器官中持续存在。
