Cesar Ilene Cristine Rosia, Soares Luís Eduardo Silva, Alves Leandro Procópio, Martin Airton Abrahão, Munin Egberto, Liporoni Priscila Christiane Suzy
Laboratory of Biomedical and Non-Linear Optics, Department of Dentistry, Vale do Paraiba University, São José dos Campos, São Paulo, Brazil.
Photomed Laser Surg. 2009 Dec;27(6):913-9. doi: 10.1089/pho.2008.2409.
To evaluate the effects of 35% hydrogen peroxide bleaching on bovine teeth using reflectance and Fourier transform (FT)-Raman spectroscopy.
Previous investigations have shown that hydrogen peroxide can modify dental components, but more studies are necessary to comprehend its effects.
Forty bovine enamel fragments (4.0x4.0x4.0 mm) were divided into four experimental groups according to the hydrogen peroxide gel manufacturer's application: G1-Whiteness HP Maxx, G2-Whiteness HP, G3-Whiteform- Perox Red Form, G4-Opalescence Xtra. All groups were activated using a light-emitting diode-laser system. The bleaching treatments were performed in two sessions with a 72-h interval between sessions. The reflectance and Raman analyses of the enamel samples were performed before and after bleaching. The analyses before treatments were used as control.
Enamel reflectance was significantly greater after the bleaching in group G3 than in groups G1, G2, and G4 (p<0.01). FT-Raman spectroscopy data showed no significant chemical changes in the inorganic components for the tested groups (p>0.05). Hydrogen peroxide gel caused significant reduction of the dental organics associated with type I collagen vibration only in group G3 (p<0.05).
Under the conditions of this study, 35% hydrogen peroxide Whiteform-Perox Red Form gel, exhibited great bleaching potential. This highly concentrated hydrogen peroxide gel significantly changed the reflectance of enamel and dental organics without significant chemical changes in enamel phosphate and carbonate content.
使用反射率和傅里叶变换（FT）-拉曼光谱法评估35%过氧化氢漂白对牛牙的影响。
先前的研究表明过氧化氢可改变牙齿成分，但需要更多研究来了解其影响。
根据过氧化氢凝胶制造商的使用方法，将40个牛牙釉质碎片（4.0×4.0×4.0毫米）分为四个实验组：G1-Whiteness HP Maxx、G2-Whiteness HP、G3-Whiteform-Perox Red Form、G4-Opalescence Xtra。所有组均使用发光二极管激光系统进行激活。漂白处理分两个阶段进行，阶段间隔为72小时。在漂白前后对釉质样本进行反射率和拉曼分析。处理前的分析用作对照。
G3组漂白后的釉质反射率显著高于G1、G2和G4组（p<0.01）。FT-拉曼光谱数据显示，测试组的无机成分无显著化学变化（p>0.05）。仅G3组的过氧化氢凝胶导致与I型胶原振动相关的牙齿有机物显著减少（p<0.05）。
在本研究条件下，35%过氧化氢Whiteform-Perox Red Form凝胶具有很大的漂白潜力。这种高浓度的过氧化氢凝胶显著改变了釉质和牙齿有机物的反射率，而釉质磷酸盐和碳酸盐含量无显著化学变化。
