Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, China.
J Hazard Mater. 2009 Dec 30;172(2-3):1282-7. doi: 10.1016/j.jhazmat.2009.07.134. Epub 2009 Aug 7.
A sensitive method for speciation analysis of inorganic mercury (Hg(2+)) and methyl mercury (MeHg(+)) has been developed by using high performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectrometry (ICP-MS) after cloud point extraction. The analytes were complexed with sodium diethyldithiocarbamate (DDTC) and preconcentrated by a non-ionic surfactant Triton X-114. Mercury species were effectively separated by HPLC in less than 6 min. The enhancement factors for 25 mL sample solution were 42 and 21, and the limits of detection were 4 and 10 ng L(-1) for Hg(2+) and MeHg(+), respectively. The developed method was successfully applied to the determination of trace amount of mercury species in environmental and biological samples.
建立了一种采用浊点萃取结合高效液相色谱-电感耦合等离子体质谱联用(HPLC-ICP-MS)测定痕量无机汞(Hg(2+))和甲基汞(MeHg(+))的形态分析方法。该方法在 pH 值为 10.0 的氨性溶液中，汞与二乙二硫代氨基甲酸钠(DDTC)形成配合物，用非离子表面活性剂 Triton X-114 萃取富集。采用 HPLC 可在 6 min 内实现汞形态的有效分离。Hg(2+)和 MeHg(+)的富集倍数分别为 42 和 21，检出限分别为 4 和 10 ng L(-1)。该方法成功地应用于环境和生物样品中痕量汞形态的测定。
