Collier A M, Peterson L P, Baseman J B
J Infect Dis. 1977 Aug;136 Suppl:S196-203. doi: 10.1093/infdis/136.supplement.s196.
Hamster tracheal organ culture was employed as a model for study of the pathogenesis of infection due to Bordetella pertussis. Infected tracheal explants were examined with light, immunofluorescence, and electrom microscopy. B. pertussis organisms preferentially attached to the ciliated cells, producing ciliostasis and marked destruction of the subcellular organelles followed by expulsion of these cells from the epithelial layer. Other nonciliated respiratory epithelial cells appeared to be unaffected. Metabolic studies on infected tracheal cultrues indicated that significant deficiencies in syntheiss of host cell protein accompanied early cytopathology. Similarities and differences in host cell and parasite interaction were noted between B. pertussis and other pathogenic agents studied in this system.
仓鼠气管器官培养被用作研究百日咳博德特氏菌感染发病机制的模型。对感染的气管外植体进行了光镜、免疫荧光和电镜检查。百日咳博德特氏菌优先附着于纤毛细胞，导致纤毛运动停止和亚细胞器显著破坏，随后这些细胞从上皮层排出。其他非纤毛呼吸道上皮细胞似乎未受影响。对感染气管培养物的代谢研究表明，宿主细胞蛋白质合成的显著缺陷伴随着早期细胞病理学变化。在该系统中研究的百日咳博德特氏菌与其他病原体之间，观察到了宿主细胞与寄生虫相互作用的异同。
