Opremcak L B, Rheins M S
Can J Microbiol. 1983 Apr;29(4):415-20. doi: 10.1139/m83-067.
Infection of mouse tracheal organ culture with Bordetella pertussis resulted in ciliostasis within 36 h. Scanning electron microscopy revealed that B. pertussis attached exclusively to ciliated cells but did not induce expulsion of this cell type at a test interval of 48 h. Mouse oviduct organ culture infected with B. pertussis demonstrated the same strict tropism for ciliated cells as in the tracheal ring system. Only ciliated cells were parasitized, becoming heavily colonized 48 h postinfection. Infected ciliated oviduct cells were not extruded. A fixation method which enhances fine structure was used in the scanning electron microscope studies. Bacterial fimbriae were not observed as the method of attachment of B. pertussis to cilia but fine fibers were seen extending between cilia and bacterial cells.
用百日咳博德特氏菌感染小鼠气管器官培养物，36小时内会导致纤毛运动停止。扫描电子显微镜显示，百日咳博德特氏菌仅附着于纤毛细胞，但在48小时的测试间隔内并未诱导这种细胞类型的排出。感染百日咳博德特氏菌的小鼠输卵管器官培养物对纤毛细胞表现出与气管环系统相同的严格嗜性。只有纤毛细胞被寄生，感染后48小时大量定植。感染的输卵管纤毛细胞未被挤出。扫描电子显微镜研究中使用了一种增强精细结构的固定方法。未观察到百日咳博德特氏菌附着于纤毛的菌毛，但可见纤毛与细菌细胞之间延伸的细纤维。
