利用 REP-PCR 技术对巴西南部污染水中的大肠杆菌进行特征描述和遗传多样性分析。
Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil.
机构信息
Departamento de Microbiologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, 900040-170 Porto Alegre, RS, Brazil.
出版信息
FEMS Microbiol Ecol. 2003 Jul 1;45(2):173-80. doi: 10.1016/S0168-6496(03)00147-8.
Abstract The scope of this study was to establish the genomic diversity existing between Escherichia coli isolates obtained from water samples retrieved from Arroio Feijó, southern Brazil, using the repetitive extragenic palindromic elements-polymerase chain reaction protocol. Ninety-eight different isolates were identified from samples obtained from five sites. Eleven different clusters presenting identical fingerprinting patterns were detected. The dendrogram contained 28 clusters for 70% similarity cut-off. These clusters enabled the observation of 16 single patterns. The results enabled the observation of genetic diversity between isolates obtained in one sampling site, and those from other sampling sites. Site 5 showed the highest diversity (Shannon-Weaver, H'=2.92) and site 3 exhibited the lowest diversity degree (Shannon-Weaver, H'=2.16).
摘要 本研究旨在利用重复扩展基因回文元件-聚合酶链反应（REP-PCR）方案，确定从巴西南部阿罗约-费霍（Arroio Feijó）水样中分离出的大肠杆菌分离株之间存在的基因组多样性。从五个地点采集的样本中鉴定出 98 个不同的分离株。检测到 11 个具有相同指纹图谱的不同聚类。聚类树状图包含 70%相似度的 28 个聚类。这些聚类可以观察到 16 个单一模式。结果表明，在一个采样点获得的分离株与其他采样点获得的分离株之间存在遗传多样性。第 5 个采样点显示出最高的多样性（Shannon-Weaver，H'=2.92），而第 3 个采样点显示出最低的多样性程度（Shannon-Weaver，H'=2.16）。
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