Pusparini Novi, Waturangi Diana E, Usia Tepy, Nikastri Eva
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Jenderal Sudirman 51, Jakarta, 12930, Indonesia.
Research Center of Drug and Food, National Agency for Drug and Food Control, Jalan Percetakan Negara 23, Jakarta, 10560, Indonesia.
BMC Res Notes. 2018 Sep 10;11(1):659. doi: 10.1186/s13104-018-3737-3.
The prevalence of Escherichia coli including from ice cubes in Indonesia is quite high. Unfortunately, little is known about the genetic diversity of E. coli from ice cube production site. Genotypic variation in E. coli populations is a major barrier to control public health risk associated with foodborne pathogen. The aims of this study were to analyze the genotypic diversity of E. coli strains isolated from various samples in order to determine the genetic relationship between those strains. This study is also important to understand the occurrence, prevalence and profile picture of different pathogenic E. coli in various sources which potentially cause disease.
Enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic polymerase chain reaction (REP-PCR) dendrogram showed high genetic diversity of 120 E. coli isolates in majority of sampling sites. DNA fingerprint patterns showed 26 and 21 clusters with 11 and 3 fingerprints individual lineages for ERIC and REP-PCR respectively. There was no correlation observed between phylogenetic relationship and virulence genes. The result indicated a variation of E. coli isolates in ice cube manufacturers. ERIC-PCR method is more discriminative compared with REP-PCR to analyze the genetic diversity of E. coli from ice cubes production sites.
在印度尼西亚，包括来自冰块中的大肠杆菌的流行率相当高。遗憾的是，关于冰块生产场所大肠杆菌的遗传多样性知之甚少。大肠杆菌群体的基因型变异是控制与食源性病原体相关的公共卫生风险的主要障碍。本研究的目的是分析从各种样本中分离出的大肠杆菌菌株的基因型多样性，以确定这些菌株之间的遗传关系。本研究对于了解不同来源中可能致病的不同致病性大肠杆菌的发生、流行情况和概况也很重要。
肠杆菌重复基因间共有序列（ERIC）和重复外显子回文聚合酶链反应（REP-PCR）树状图显示，在大多数采样点，120株大肠杆菌分离株具有高度的遗传多样性。DNA指纹图谱分别显示了26个和21个簇，ERIC和REP-PCR分别有11个和3个指纹单独谱系。未观察到系统发育关系与毒力基因之间的相关性。结果表明冰块制造商处的大肠杆菌分离株存在变异。与REP-PCR相比，ERIC-PCR方法在分析来自冰块生产场所的大肠杆菌的遗传多样性方面更具鉴别力。
