Abdelwahab Adel A, Lee Hak-Myoung, Shim Yoon-Bo
Department of Chemistry and Center for Innovative BioPhysio Sensor Technology, Pusan National University, Busan 609-735, South Korea.
Anal Chim Acta. 2009 Sep 21;650(2):247-53. doi: 10.1016/j.aca.2009.07.054. Epub 2009 Jul 28.
A selective detection method for dopamine (DA) was developed by incorporating cibacron blue (F3GA) into poly-1,5-diaminonaphthalene (PDAN) layer. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry (CV) were employed to characterize the modified surfaces. The modified electrode was effective in selectively facilitating the electron transfer of DA and blocking the interferences of negatively charged species attributed to the sulfonate groups in the F3GA/PDAN composite film. This method enabled the determination of DA in the presence of various interfering species, including ascorbic acid (AA), in a phosphate buffer solution (pH 7.4). The modified electrode demonstrated good performance in the detection of DA in a concentration range of 5.0-100 microM, with a detection limit (k=3) of 0.1+/-0.01 microM. The application was conducted for the determination of DA in a human urine sample and the sensor was proven to be rapid, has excellent selectivity, and stable amperometric response.
通过将汽巴克隆蓝（F3GA）掺入聚-1,5-二氨基萘（PDAN）层中，开发了一种多巴胺（DA）的选择性检测方法。采用扫描电子显微镜（SEM）、X射线光电子能谱（XPS）和循环伏安法（CV）对修饰表面进行表征。修饰电极有效地选择性促进了DA的电子转移，并阻断了F3GA/PDAN复合膜中磺酸根基团引起的带负电物质的干扰。该方法能够在含有各种干扰物质（包括抗坏血酸（AA））的磷酸盐缓冲溶液（pH 7.4）中测定DA。修饰电极在5.0 - 100 microM的DA浓度范围内检测表现良好，检测限（k = 3）为0.1±0.01 microM。该方法应用于人体尿液样本中DA的测定，结果表明该传感器具有快速、优异的选择性和稳定的安培响应。
