Systems and Process Engineering Centre (SPEC), Centre for NanoHealth, College of Engineering, Swansea University, Swansea, SA2 8PP, UK.
Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing 400044, China.
Biosensors (Basel). 2019 Jan 18;9(1):16. doi: 10.3390/bios9010016.
Grafting thin polymer layers on graphene enables coupling target biomolecules to graphene surfaces, especially through amide and aldehyde linkages with carboxylic acid and primary amine derivatives, respectively. However, functionalizing monolayer graphene with thin polymer layers without affecting their exceptional electrical properties remains challenging. Herein, we demonstrate the controlled modification of chemical vapor deposition (CVD) grown single layer graphene with ultrathin polymer 1,5-diaminonaphthalene (DAN) layers using the electropolymerization technique. It is observed that the controlled electropolymerization of DAN monomer offers continuous polymer layers with thickness ranging between 5⁻25 nm. The surface characteristics of pure and polymer modified graphene was examined. As anticipated, the number of surface amine groups increases with increases in the layer thickness. The effects of polymer thickness on the electron transfer rates were studied in detail and a simple route for the estimation of surface coverage of amine groups was demonstrated using the electrochemical analysis. The implications of grafting ultrathin polymer layers on graphene towards horseradish peroxidase (HRP) enzyme immobilization and enzymatic electrochemical sensing of H₂O₂ were discussed elaborately.
在石墨烯上嫁接薄聚合物层可将靶标生物分子偶联到石墨烯表面，特别是通过酰胺和醛键分别与羧酸和伯胺衍生物偶联。然而，在不影响其优异的电性能的情况下，用薄聚合物层功能化单层石墨烯仍然具有挑战性。在此，我们展示了使用电化学聚合技术对化学气相沉积（CVD）生长的单层石墨烯进行超薄膜聚合物 1,5-二氨基萘（DAN）层的可控修饰。观察到 DAN 单体的受控电化学聚合提供了厚度在 5⁻25nm 之间的连续聚合物层。检查了纯石墨烯和聚合物修饰石墨烯的表面特性。正如预期的那样，表面胺基的数量随层厚度的增加而增加。详细研究了聚合物厚度对电子转移速率的影响，并通过电化学分析证明了一种简单的估算胺基团表面覆盖率的方法。详细讨论了在石墨烯上嫁接超薄聚合物层对辣根过氧化物酶（HRP）酶固定化和 H₂O₂酶电化学传感的影响。
