从1型糖尿病患者中生成多能干细胞。
Generation of pluripotent stem cells from patients with type 1 diabetes.
作者信息
Maehr René, Chen Shuibing, Snitow Melinda, Ludwig Thomas, Yagasaki Lisa, Goland Robin, Leibel Rudolph L, Melton Douglas A
机构信息
Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
出版信息
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15768-73. doi: 10.1073/pnas.0906894106. Epub 2009 Aug 31.
Abstract
Type 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic beta cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallmarks of pluripotency and can be differentiated into insulin-producing cells. These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy.
摘要
1型糖尿病（T1D）是胰腺β细胞发生自身免疫性破坏的结果。导致该疾病的细胞和分子缺陷仍不清楚。从T1D患者产生的多能细胞将有助于疾病建模。我们在此表明，通过用三种转录因子（OCT4、SOX2、KLF4）对成年成纤维细胞进行重编程，可从T1D患者产生诱导多能干细胞（iPS细胞）。T1D特异性iPS细胞，即DiPS细胞，具有多能性的特征，并且可以分化为产生胰岛素的细胞。这些结果朝着在T1D疾病建模以及细胞替代治疗中使用DiPS细胞迈出了一步。
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