Yamashita Daisuke, Machigashira Miho, Miyamoto Motoharu, Takeuchi Hironobu, Noguchi Kazuyuki, Izumi Yuichi, Ban Seiji
Department of Periodontology, Graduate school of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Dent Mater J. 2009 Jul;28(4):461-70. doi: 10.4012/dmj.28.461.
The aim of this study was to evaluate the effect of surface roughness on the initial attachment of mouse osteoblast-like cells on ceriastabilized zirconia/alumina nanocomposite (NANOZR) and yttria-stabilized zirconia (3Y-TZP) in comparison to those on pure titanium (Ti) and alumina oxide (AO). Specimens with smooth and rough surfaces were prepared by grinding with diamond paper or by sandblasting, respectively. For four substrates examined, the number of attached cells on the rough surface specimens was significantly higher than that on the smooth surface specimens (p < 0.05). Integrin alpha(5) and beta(1) expression had a greater increase in rough surface specimens than in smooth surface specimens. Actin cytoskeleton organization was, however, similar for both smooth and rough surface specimens. NANOZR and 3Y-TZP produced good cell attachment, similar to Ti and AO. The overall results demonstrated that NANOZR and 3Y-TZP with rough surface could provide good initial cell responses, adequate for future implant usage.
本研究的目的是评估表面粗糙度对小鼠成骨样细胞在铈稳定氧化锆/氧化铝纳米复合材料（NANOZR）和氧化钇稳定氧化锆（3Y-TZP）上的初始附着的影响，并与在纯钛（Ti）和氧化铝（AO）上的情况进行比较。分别通过用金刚石砂纸研磨或喷砂制备了具有光滑和粗糙表面的样本。对于所检测的四种基底，粗糙表面样本上附着的细胞数量显著高于光滑表面样本（p < 0.05）。整合素α(5)和β(1)的表达在粗糙表面样本中的增加幅度大于光滑表面样本。然而，光滑和粗糙表面样本的肌动蛋白细胞骨架组织相似。NANOZR和3Y-TZP产生了良好的细胞附着，与Ti和AO相似。总体结果表明，具有粗糙表面的NANOZR和3Y-TZP能够提供良好的初始细胞反应，足以用于未来的植入应用。
