Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Setor Universitário, 74605-050 Goiânia, Brasil.
Mem Inst Oswaldo Cruz. 2009 Jul;104(4):655-8. doi: 10.1590/s0074-02762009000400021.
Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested RT-PCR and/or genomic sequencing. HAstV-1 (42.8%), HAstV-2 (23.2%), HAstV-3 (3.6%), HAstV-4 (14.3%) and HAstVs -5, -6, -7 and -8 (1.8% each) were detected. In Goiânia and Campo Grande, HAstV-1 was the most frequently detected genotype while in Brasília (DF) it was HAstV-2. Shifts in the circulation of astrovirus genotypes were observed in DF and Campo Grande. All samples collected by rectal swabs were viral negative. The astrovirus genotypes were detected in all age groups and there was no correlation between genotype and age group.
从巴西中西部三个地区采集的 1588 份儿童粪便样本中，使用逆转录-聚合酶链反应（RT-PCR）检测到 57 份星状病毒（HAstVs）阳性。通过巢式 RT-PCR 和/或基因组测序对其进行了基因分型。检测到 HAstV-1（42.8%）、HAstV-2（23.2%）、HAstV-3（3.6%）、HAstV-4（14.3%）和 HAstVs-5、-6、-7 和 -8（各 1.8%）。在戈亚尼亚和坎波格兰德，HAstV-1 是最常检测到的基因型，而在巴西利亚（DF）则是 HAstV-2。在 DF 和坎波格兰德观察到星状病毒基因型的循环变化。所有通过直肠拭子采集的样本均为病毒阴性。在所有年龄组中均检测到星状病毒基因型，且基因型与年龄组之间无相关性。
