Resque Hugo Reis, Munford Veridiana, Castilho Juliana Galera, Schmich Herika, Caruzo Thabata Alessandra Ramos, Rácz Maria Lucia
Laboratório de Gastroenterites Virais, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz. 2007 Dec;102(8):969-74. doi: 10.1590/s0074-02762007000800012.
The purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in São Paulo, Brazil, grouped into two sets: EPM and HU. Detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (RT-PCR) with specific primers directed towards the genome open reading frame 2 (ORF2). Results for EPM set showed that 66/234 (28.2%) were positive: 28/94 (29.7%) from children with acute diarrhea, 14/45 (31.1%) with persistent diarrhea, and 9/55 (16.3%) from control individuals. No data was available for 15/40 (37.5%) of samples. Mixed infections with other viruses were found in 33 samples. In the HU, 18/187 (9.6%) were positive: 12/158 (7.6%) from individuals with acute diarrhea and 6/29 (20.7%) from control children. Four samples were mixed with other viruses. Out of 66 astrovirus positive EPM samples, 18 (27.2%) were characterized as human astrovirus type-1 (HAstV-1), two (3.0%) as HAstV-2, two (3.0%) as HAstV-3, and three (4.5%) as HAstV-8. Among 18 astrovirus positive HU samples, one (5.5%) was characterized as HAstV-1, six (33.3%) as HAstV-2, and one (5.5%) as HAstV-8. Two HAstV-8 genotyped samples were further confirmed by nucleotide sequencing. Our results shows that astroviruses are circulating in a constant manner in the population, with multiple serotypes, in higher frequency than it was described for other Brazilian regions. For the first time in Sao Paulo, Brazil, it was shown that astroviruses play an important role in children gastroenteritis, as described for most locations where they were detected.
本研究的目的是对从巴西圣保罗腹泻和未腹泻儿童粪便样本中分离出的星状病毒进行特征分析，这些样本分为两组：EPM组和HU组。采用针对基因组开放阅读框2（ORF2）的特异性引物，通过逆转录巢式聚合酶链反应（RT-PCR）进行检测和基因分型。EPM组的结果显示，66/234（28.2%）呈阳性：急性腹泻儿童中28/94（29.7%），持续性腹泻儿童中14/45（31.1%），对照个体中9/55（16.3%）。15/40（37.5%）的样本无数据。33个样本中发现与其他病毒的混合感染。在HU组中，18/187（9.6%）呈阳性：急性腹泻个体中12/158（7.6%），对照儿童中6/29（20.7%）。4个样本与其他病毒混合。在66个星状病毒阳性的EPM样本中，18个（27.2%）被鉴定为人星状病毒1型（HAstV-1），2个（3.0%）为HAstV-2，2个（3.0%）为HAstV-3，3个（4.5%）为HAstV-8。在18个星状病毒阳性的HU样本中，1个（5.5%）被鉴定为HAstV-1，6个（33.3%）为HAstV-2，1个（5.5%）为HAstV-8。两个HAstV-8基因分型样本通过核苷酸测序进一步确认。我们的结果表明，星状病毒在人群中持续传播，有多种血清型，其频率高于巴西其他地区的报道。在巴西圣保罗首次表明，星状病毒在儿童胃肠炎中起重要作用，正如在大多数检测到它们的地方所描述的那样。
