Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA.
Colloids Surf B Biointerfaces. 2011 Jul 1;85(2):168-73. doi: 10.1016/j.colsurfb.2011.02.024. Epub 2011 Feb 22.
In this work, particle immunoagglutination assays for pathogen detection, utilizing light scattering measurements at a fixed angle from incident light delivery, are explored in both Rayleigh and Mie scatter regimes through scatter intensity simulations and compared to experimental results. The average size of immunoagglutinated particles obtained from microscope images correspond to the particle size parameter from simulations. Mie scatter measurements yield a greater signal increase with increasing pathogen concentration than Rayleigh scatter measurements, but with a non-monotonic relationship that is not observed in the Rayleigh scatter regime. These two similar yet distinctly different sources of information could easily be integrated into a single device through fabrication of a simple microfluidic device containing two y-channels, each for performing the respective light scattering measurement. Escherichia coli was used as a representative target, and detected in a microfluidic device down to a concentration of 1 colony forming units (CFU) per mL.
在这项工作中，通过散射强度模拟，研究了在瑞利和米氏散射区域中利用从入射光传输固定角度进行的光散射测量的用于病原体检测的颗粒免疫凝集分析，并与实验结果进行了比较。从显微镜图像获得的免疫凝集颗粒的平均大小与模拟中的颗粒大小参数相对应。与瑞利散射测量相比，米氏散射测量随着病原体浓度的增加而产生更大的信号增加，但不存在瑞利散射区域中观察到的非单调关系。这两种相似但明显不同的信息源可以通过制造一个简单的微流控装置轻松地集成到单个设备中，该装置包含两个 y 通道，每个通道用于进行相应的光散射测量。大肠杆菌被用作代表性靶标，并在微流控装置中检测到每毫升 1 个菌落形成单位 (CFU) 的浓度。
