Scovassi A Ivana, Soldani Cristiana, Veneroni Paola, Bottone Maria Grazia, Pellicciari Carlo
Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Pavia, Italy.
Ann N Y Acad Sci. 2009 Aug;1171:12-7. doi: 10.1111/j.1749-6632.2009.04707.x.
During apoptosis, apoptosis-inducing factor (AIF) is released from the mitochondrial intermembrane space to the cytosol and to the nucleus. We analyzed AIF in HeLa cells driven to apoptosis by either etoposide or actinomycin D, and we observed changes in the structure and function of mitochondria as well as the translocation of cytochrome c and AIF from mitochondria to the nucleus in early apoptosis. In cells with fragmented chromatin (i.e., in late apoptosis), the immunolabeling for AIF appeared to be distinct from chromatin, being mainly confined to mitochondria.
在细胞凋亡过程中，凋亡诱导因子（AIF）从线粒体膜间隙释放到细胞质和细胞核中。我们分析了经依托泊苷或放线菌素D诱导发生凋亡的HeLa细胞中的AIF，并且观察到在早期凋亡过程中线粒体的结构和功能发生变化，以及细胞色素c和AIF从线粒体向细胞核的转位。在染色质碎片化的细胞中（即晚期凋亡细胞），AIF的免疫标记似乎与染色质不同，主要局限于线粒体。
