Henricks P A, Binkhorst G J, Drijver A A, Van der Vliet H, Nijkamp F P
Department of Pharmacology, Faculty of Pharmacy, State University of Utrecht, The Netherlands.
Vet Microbiol. 1990 Apr;22(2-3):259-66. doi: 10.1016/0378-1135(90)90113-a.
It was investigated whether beta-adrenoceptor antagonists could disturb the interaction between cytotoxin preparations isolated from Pasteurella haemolytica and bovine polymorphonuclear leukocytes (PMNs). The toxicity of the cytotoxin preparation was evaluated by measuring the chemiluminescence response and the viability of the cells after incubation with the cytotoxin. No effect on cell viability was detected when PMNs were incubated with 63 micrograms cytotoxin per ml while the chemiluminescence response was diminished by approximately 30%. The beta-adrenoceptor antagonists alprenolol (10(-5) M) and propranolol (5 X 10(-7) - 5 X 10(-6) M) were able to attenuate this effect of cytotoxin on the chemiluminescence response of PMNs. It seemed unlikely that propranolol and alprenolol diminished the effect of cytotoxin on the chemiluminescence response of PMNs by their beta-adrenoceptor blocking potency because other beta-adrenoceptor antagonists used were without effect. Also, the membrane stabilizing characteristics of the beta-adrenoceptor antagonists used were probably not responsible for the diminished interaction between PMNs and the cytotoxin. Whether beta-adrenoceptor antagonists could be used in vivo to prevent or treat P. haemolytica infections in bovines remains to be examined.
研究了β-肾上腺素能受体拮抗剂是否会干扰从溶血巴氏杆菌中分离出的细胞毒素制剂与牛多形核白细胞（PMN）之间的相互作用。通过测量化学发光反应以及细胞毒素孵育后细胞的活力来评估细胞毒素制剂的毒性。当PMN与每毫升63微克细胞毒素孵育时，未检测到对细胞活力的影响，而化学发光反应降低了约30%。β-肾上腺素能受体拮抗剂阿普洛尔（10^(-5) M）和普萘洛尔（5×10^(-7) - 5×10^(-6) M）能够减弱细胞毒素对PMN化学发光反应的这种影响。普萘洛尔和阿普洛尔不太可能通过其β-肾上腺素能受体阻断效力来减弱细胞毒素对PMN化学发光反应的影响，因为所使用的其他β-肾上腺素能受体拮抗剂没有效果。此外，所使用的β-肾上腺素能受体拮抗剂的膜稳定特性可能也不是PMN与细胞毒素之间相互作用减弱的原因。β-肾上腺素能受体拮抗剂是否可用于牛体内预防或治疗溶血巴氏杆菌感染仍有待研究。
