O'Brien J K, Duffus W P
Vet Microbiol. 1987 Apr;13(4):321-34. doi: 10.1016/0378-1135(87)90063-0.
An in vitro 51Cr-release assay was used to compare the susceptibility of various leucocytes from normal cattle to Pasteurella haemolytica cytotoxin. Neutrophils were found to be more sensitive than mammary or bronchoalveolar macrophages. Neutrophils induced with lipopolysaccharide (LPS) and mammary macrophages activated in vitro with LPS were as sensitive as homologous untreated cells. Bronchoalveolar macrophages from adult cows were significantly more resistant than those from calves. Sub-cytolytic concentrations of cytotoxin did not impair killing of para-influenza-3 virus infected MDBK cells by mammary macrophages.
采用体外51Cr释放试验比较正常牛的各种白细胞对溶血巴氏杆菌细胞毒素的敏感性。发现嗜中性粒细胞比乳腺或支气管肺泡巨噬细胞更敏感。用脂多糖（LPS）诱导的嗜中性粒细胞和在体外被LPS激活的乳腺巨噬细胞与未处理的同源细胞一样敏感。成年母牛的支气管肺泡巨噬细胞比犊牛的更具抗性。细胞毒素的亚溶细胞浓度不会损害乳腺巨噬细胞对副流感3病毒感染的MDBK细胞的杀伤作用。
