Yu Ji Hoon, Kang Sin-Gun, Jung Un-Young, Jun Chul-Ho, Kim Hyeyoung
Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul, Korea.
Ann N Y Acad Sci. 2009 Aug;1171:359-64. doi: 10.1111/j.1749-6632.2009.04703.x.
Oxidative stress plays a critical role in apoptosis of gastric epithelial cells. Omega-3 fatty acids show anti-inflammatory and/or anticancer effects and regulate apoptosis in various cells. In this study, we aimed to investigate whether omega-3 fatty acids inhibit oxidative stress-induced apoptosis of gastric epithelial cells. The cells received oxidative stress caused by silica-immobilized glucose oxidase acting on beta-D-glucose and cultured in the absence or presence of alpha-linolenic acid or docosahexanoic acid. Viable cell numbers, levels of H(2)O(2) in the medium, DNA fragmentation, and protein levels of p53 and Bax were determined. As a result, silica-immobilized glucose oxidase acting on beta-D-glucose consistently and reproducibly produced H(2)O(2), which decreased cell viability and increased DNA fragmentation of the cells. Omega-3 fatty acids inhibited oxidative stress-induced cell death, DNA fragmentation, and induction of p53 and Bax of the cells. The silica-immobilized glucose oxidase could be a useful tool for studies on oxidative stress-induced cellular events because it is reusable and forms a stable enzyme system acting on glucose. Omega-3 fatty acids may be beneficial for preventing oxidative stress-induced apoptosis by inhibiting apoptotic gene expression and DNA fragmentation of gastric epithelial cells.
氧化应激在胃上皮细胞凋亡中起关键作用。ω-3脂肪酸具有抗炎和/或抗癌作用，并能调节多种细胞的凋亡。在本研究中，我们旨在探讨ω-3脂肪酸是否能抑制氧化应激诱导的胃上皮细胞凋亡。细胞受到固定在二氧化硅上的葡萄糖氧化酶作用于β-D-葡萄糖所引起的氧化应激，并在不存在或存在α-亚麻酸或二十二碳六烯酸的情况下进行培养。测定了活细胞数量、培养基中H(2)O(2)水平、DNA片段化以及p53和Bax的蛋白质水平。结果显示，固定在二氧化硅上的葡萄糖氧化酶作用于β-D-葡萄糖能持续且可重复地产生H(2)O(2)，这降低了细胞活力并增加了细胞的DNA片段化。ω-3脂肪酸抑制了氧化应激诱导的细胞死亡、DNA片段化以及细胞中p53和Bax的诱导。固定在二氧化硅上的葡萄糖氧化酶可能是研究氧化应激诱导的细胞事件的有用工具，因为它可重复使用并形成作用于葡萄糖的稳定酶系统。ω-3脂肪酸可能通过抑制胃上皮细胞的凋亡基因表达和DNA片段化，对预防氧化应激诱导的凋亡有益。
