Nikiforova I F, Serova O M, Pluzhnikova G F, Kniazev P G, Seĭts I F
Genetika. 1990 Mar;26(3):531-40.
The c-Ha-ras-1 locus in 84 cancer patients was examined for allelic restriction fragment length's polymorphism, as well as for distribution of four common c-Ha-ras-1 alleles (a1, a2, a3 and a4) in lung, ovarian and thyroid cancer patients. In approximately half (8 out of 15) lung and ovarian carcinomas possessing a4 allele, alterations in the Ha-ras locus (deletion, amplification and change in allele length) were detected, as compared to 2 cases of rearrangements out of 40 tumors lacking the a4 allele. An increased a4 allele frequency was found in individuals with lung and ovarian carcinomas, as compared to both controls--summarized literature data, and thyroid cancer patients. On the other hand, homozygosity for the a2 locus, resulting from deletion in another allele, and increased a2 allele frequency in thyroid cancer patients were observed. Thus, a4 and a2 alleles of the c-Ha-ras-1 may perhaps be viewed as genetic markers of predisposition to lung, ovarian and thyroid cancer, respectively, in combination with other clinical parameters.
对84例癌症患者的c-Ha-ras-1基因座进行了等位基因限制性片段长度多态性检测，以及在肺癌、卵巢癌和甲状腺癌患者中四种常见c-Ha-ras-1等位基因（a1、a2、a3和a4）的分布检测。在大约一半（15例中有8例）携带a4等位基因的肺癌和卵巢癌中，检测到Ha-ras基因座的改变（缺失、扩增和等位基因长度变化），而在40例缺乏a4等位基因的肿瘤中，有2例发生了重排。与对照组（汇总文献数据）和甲状腺癌患者相比，肺癌和卵巢癌患者中a4等位基因频率增加。另一方面，观察到甲状腺癌患者中由于另一个等位基因缺失导致a2基因座纯合，且a2等位基因频率增加。因此，结合其他临床参数，c-Ha-ras-1的a4和a2等位基因可能分别被视为肺癌、卵巢癌和甲状腺癌易感性的遗传标志物。
