c-Ha-ras-1 polymorphism in human breast carcinomas: evidence for a normal distribution of alleles.

Analysis of the c-Ha-ras-1 locus for restriction fragment length polymorphism was determined by Southern blot hybridization in 112 specimens of primary breast carcinoma. Normal cell samples were lymphocytes obtained from 61 of these breast cancer patients and from 62 healthy donors. Our data indicate that the distribution of c-Ha-ras-1 alleles in breast carcinoma patients did not significantly differ from that found in normal individuals. In addition the loss of one allele detected in 5 tumor samples was not correlated to the aggressiveness of breast cancer. Consequently our data imply that hereditary predisposition to breast cancer is not associated with the c-Ha-ras-1 polymorphism as previously reported.