Lee J H, Kavanagh J J, Wharton J T, Wildrick D M, Blick M
Department of Medical Oncology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Cancer Res. 1989 Mar 1;49(5):1220-2.
Recent reports have shown allele loss at the c-Ha-ras1 locus on the short arm of chromosome 11 in some types of tumors. To determine whether loss of heterozygosity occurs at the c-Ha-ras1 locus in uncultured human ovarian carcinomas we used Southern blot analysis to study DNA from 17 pairs of ovarian tumors and matched white blood cell samples from the same patients. In one of these 17 tumors, the c-Ha-ras1 locus was rearranged, and in five tumor DNAs from ten informative patients, a c-Ha-ras1 allele was lost. This loss, of relatively high incidence, appears to be an important characteristic of human ovarian cancer and may provide a useful tool for understanding its biological behavior.
最近的报告显示，在某些类型的肿瘤中，11号染色体短臂上的c-Ha-ras1基因座存在等位基因缺失。为了确定未培养的人类卵巢癌中c-Ha-ras1基因座是否发生杂合性缺失，我们使用Southern印迹分析来研究17对卵巢肿瘤的DNA以及来自同一患者的匹配白细胞样本。在这17个肿瘤中的1个中，c-Ha-ras1基因座发生了重排，在来自10名信息丰富患者的5个肿瘤DNA中，一个c-Ha-ras1等位基因缺失。这种发生率相对较高的缺失似乎是人类卵巢癌的一个重要特征，可能为理解其生物学行为提供一个有用的工具。
