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用于啮齿动物和人类少突胶质细胞特化、谱系进展和成熟的培养系统。

Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.

作者信息

Espinosa-Jeffrey Araceli, Wakeman Dustin R, Kim Seung U, Snyder Evan Y, de Vellis Jean

机构信息

David Geffen School of Medicine at UCLA, Los Angeles, California, USA.

出版信息

Curr Protoc Stem Cell Biol. 2009 Sep;Chapter 2:Unit 2D.4. doi: 10.1002/9780470151808.sc02d04s10.

DOI:10.1002/9780470151808.sc02d04s10
PMID:19725014
Abstract

Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.

摘要

在此，我们记录了从啮齿动物和人类神经干细胞中产生、分离和维持少突胶质细胞表型的方案。我们独特的方法依赖于一系列化学成分明确的培养基，这些培养基是专门为少突胶质细胞从少突胶质前体细胞发育为成熟的髓鞘形成少突胶质细胞的每个发育阶段设计并经过仔细表征的。

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