少突胶质细胞的分离与培养
Isolation and Culture of Oligodendrocytes.
作者信息
Weil Marie-Theres, Schulz-Ëberlin Giselheid, Mukherjee Chaitali, Kuo-Elsner Wen Ping, Schäfer Isabelle, Müller Christina, Simons Mikael
机构信息
Max Planck Institute of Experimental Medicine, Goettingen, Germany.
Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Goettingen, Germany.
出版信息
Methods Mol Biol. 2019;1936:79-95. doi: 10.1007/978-1-4939-9072-6_5.
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.
源自啮齿动物大脑的原代细胞培养物被广泛用于研究神经生物学中的分子和细胞机制。在本章中，我们描述了从小鼠围产期大脑中纯化和培养少突胶质细胞的方法。此外，我们还描述了将纯化的少突胶质细胞与神经元共培养的方法。按照这些方案制备和培养时，可以在培养中研究少突胶质细胞生物学的许多重要方面，例如它们的增殖、分化和髓鞘形成。
Zotero 插件