Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France.
Magn Reson Med. 2009 Oct;62(4):840-54. doi: 10.1002/mrm.22085.
The purpose of the present study was to assess the reliability of metabolic parameters measured using (31)P magnetic resonance spectroscopy ((31)P MRS) during two standardized rest-exercise-recovery protocols. Twelve healthy subjects performed the standardized protocols at two different intensities; i.e., a moderate intensity (MOD) repeated over a two-month period and heavy intensity (HEAVY) repeated over a year's time. Test-retest reliability was analyzed using coefficient of variation (CV), limits of agreement (LOA), and intraclass correlation coefficients (ICC). During exercise and recovery periods, most of the metabolic parameters exhibited a good reliability. The CVs of individual concentration of phosphocreatine ([PCr]), concentration of adenosine diphosphate ([ADP]), and pH values recorded at end of the HEAVY exercise were lower than 15%. The CV calculated for the rate of PCr resynthesis and the maximal oxidative capacity were less than 13% during the HEAVY protocol. Inferred parameters such as oxidative and total adenosine triphosphate (ATP) production rates exhibited a good reliability (ICC approximately 0.7; CV < 15% during the HEAVY protocol). Our results demonstrated that measurement error using (31)P-MRS during a standardized exercise was low and that biological variability accounted for the vast majority of the measurement variability. In addition, the corresponding metabolic measurements can reliably be used for longitudinal studies performed even over a long period of time.
本研究旨在评估在两种标准化的休息-运动-恢复方案中使用（31）P 磁共振波谱（（31）P MRS）测量的代谢参数的可靠性。12 名健康受试者以两种不同的强度进行了标准化方案；即在两个月内重复进行中度强度（MOD），并在一年内重复进行高强度（HEAVY）。使用变异系数（CV）、协议范围（LOA）和组内相关系数（ICC）分析测试-再测试的可靠性。在运动和恢复期间，大多数代谢参数表现出良好的可靠性。记录的 HEAVY 运动结束时磷酸肌酸（[PCr]）的个体浓度、二磷酸腺苷（[ADP]）浓度和 pH 值的 CV 低于 15%。在 HEAVY 方案中，PCr 再合成率和最大氧化能力的 CV 计算值低于 13%。推断参数如氧化和总三磷酸腺苷（ATP）产生率表现出良好的可靠性（ICC 约为 0.7；在 HEAVY 方案中 CV<15%）。我们的结果表明，使用（31）P-MRS 在标准化运动期间的测量误差较低，生物变异性占测量变异性的绝大部分。此外，即使在很长一段时间内进行纵向研究，相应的代谢测量也可以可靠地使用。
