Barbato O, Sousa N M, Debenedetti A, Canali C, Todini L, Beckers J F
Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy.
Theriogenology. 2009 Oct 15;72(7):993-1000. doi: 10.1016/j.theriogenology.2009.06.020. Epub 2009 Sep 1.
The aim of the current study was to describe the use of a pool of different antisera raised against pregnancy-associated glycoproteins (PAGs; purified from both ovine and caprine placentas) for early pregnancy diagnosis in ovine species. Sixty-three pluriparous Sarda ewes (Ovis aries) were synchronized. Blood samples were withdrawn on Days 18, 24, 26, 28, 30, and 50 after mating. These samples were assayed for progesterone (radioimmunoassay [RIA] including an extraction step) and for pregnancy-associated glycoproteins (RIA-706 and RIA-srPool). Progesterone concentrations were under 1.0 ng/mL in all nonpregnant Sarda ewes. In pregnant ewes, mean progesterone concentrations ranged from 2.4 ng/mL (Day 24, single pregnancies) to 4.4 ng/mL (Day 28, multiple pregnancies). During all periods of examination, PAGs remained lower than 0.8 ng/mL in nonpregnant ewes. On Day 18 of pregnancy, PAG concentrations could be detected in 26 of 43 (60.5%) and in 41 of 43 (95.3%) pregnant ewes using the RIA-706 and RIA-srPool methods, respectively. From Day 24 to Day 50, using both RIA methods, PAGs could be detected in all pregnant ewes. On Day 24, the best threshold for pregnancy diagnosis was obtained by use of RIA-srPool, maximal concentration in nonpregnant ewes being 0.3 ng/mL and minimal concentration in pregnant ewes being 4.8 ng/mL. In general, progesterone and PAG concentrations were higher in multiple pregnancies than in single pregnancies. However, because of large individual variations, single pregnancies could not be differentiated from multiple pregnancies.
本研究的目的是描述使用一组针对妊娠相关糖蛋白（PAGs；从绵羊和山羊胎盘纯化而来）制备的不同抗血清，用于绵羊早期妊娠诊断。对63只经产撒丁岛母羊（绵羊）进行了同期发情处理。在配种后的第18、24、26、28、30和50天采集血样。这些样本用于检测孕酮（包括提取步骤的放射免疫分析[RIA]）和妊娠相关糖蛋白（RIA - 706和RIA - srPool）。所有未怀孕的撒丁岛母羊孕酮浓度均低于1.0 ng/mL。怀孕母羊的平均孕酮浓度范围为2.4 ng/mL（第24天，单胎妊娠）至4.4 ng/mL（第28天，多胎妊娠）。在所有检查期间，未怀孕母羊的PAGs浓度均低于0.8 ng/mL。在妊娠第18天，分别使用RIA - 706和RIA - srPool方法，在43只怀孕母羊中的26只（60.5%）和41只（95.3%）中检测到了PAG浓度。从第24天到第50天，使用两种RIA方法，在所有怀孕母羊中均检测到了PAGs。在第24天，使用RIA - srPool获得了最佳的妊娠诊断阈值，未怀孕母羊的最大浓度为0.3 ng/mL，怀孕母羊的最小浓度为4.8 ng/mL。一般来说，多胎妊娠的孕酮和PAG浓度高于单胎妊娠。然而，由于个体差异较大，单胎妊娠和多胎妊娠无法区分。
