Wang Chun-xia, Zeng Xu-xin, Huang Le-song, Hou Lian-bing
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Aug;29(8):1541-4.
To investigate the effect of Changtong oral liquid (CTOL) on the proliferation of cultured fibroblasts derived from normal peritoneum (NFs) and adhesive peritoneum (AFs) of rats.
Twenty male SD rats were randomized into 4 groups, including a normal serum group and 3 CTOL groups with CTOL treatment at low, medium or high doses. Serum samples were obtained from the abdominal arteries of the rats after oral treatment with CTOL for 7 days. The fibroblasts were isolated from the peritoneum by means of tissue culture, and the passage 3-8 cells were cultured with the sera of the normal control and CTOL groups for 24, 48, 72 and 96 h. MTT assay was used to observe the proliferation of the fibroblasts.
The dose of CTOL was inversely correlated to the absorbance but positively to the growth inhibition rates. Compared with the NFs cultured in normal control rat serum, the NFs in serum from CTOL groups showed no obviously changes in the absorbance at 24 and 48 h, but displayed significant reduction at 72 and 96 h (P<0.01). Compared with the AFs in normal rat serum, the AFs in the 3 CTOL groups all showed significantly decreased absorbance at 24, 48, 72 and 96 h (P<0.05). At the same time point, the inhibition rate of AFs in low-dose CTOL group showed no significant difference from that in the normal control group, but CTOL at a medium dose resulted in a significantly higher inhibition rate of AFs at 72 h (P<0.05). High-dose CTOL produced significant differences in the inhibition rates of AFs and NFs (P<0.05).
CTOL can inhibit the proliferation of AFs and NFs in vitro. AFs appear to be more sensitive to CTOL, which has a dose-dependent inhibitory effect of AF proliferation.
探讨肠通口服液（CTOL）对大鼠正常腹膜成纤维细胞（NFs）和粘连腹膜成纤维细胞（AFs）增殖的影响。
将20只雄性SD大鼠随机分为4组，包括正常血清组和3个CTOL组，分别给予低、中、高剂量CTOL治疗。CTOL灌胃治疗7天后，从大鼠腹主动脉采集血清样本。采用组织培养法从腹膜分离成纤维细胞，将第3 - 8代细胞与正常对照组和CTOL组血清分别培养24、48、72和96小时。采用MTT法观察成纤维细胞的增殖情况。
CTOL剂量与吸光度呈负相关，与生长抑制率呈正相关。与正常对照大鼠血清培养的NFs相比，CTOL组血清培养的NFs在24和48小时吸光度无明显变化，但在72和96小时显著降低（P<0.01）。与正常大鼠血清培养的AFs相比，3个CTOL组的AFs在24、48、72和96小时吸光度均显著降低（P<0.05）。在同一时间点，低剂量CTOL组AFs的抑制率与正常对照组无显著差异，但中剂量CTOL在72小时导致AFs抑制率显著升高（P<0.05）。高剂量CTOL对AFs和NFs的抑制率有显著差异（P<0.05）。
CTOL可体外抑制AFs和NFs的增殖。AFs对CTOL似乎更敏感，CTOL对AFs增殖具有剂量依赖性抑制作用。
