定位在兔痘病毒和痘苗病毒DNA上的HindIII和Sst I限制性酶切位点。

Wittek R, Menna A, Schümperli D, Stoffel S, Müller H K, Wyler R

J Virol. 1977 Sep;23(3):669-78. doi: 10.1128/JVI.23.3.669-678.1977.

The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.

利用限制性内切酶HindIII和Sst I，通过重叠片段分析对两种密切相关的正痘病毒——兔痘病毒（RPV）和痘苗病毒（VV）的DNA进行了图谱绘制。这些片段的确切排列是通过对分离的部分限制性产物进行完全酶切以及末端片段测定来完成的。RPV和VV DNA在包含约60%基因组的内部区域显示出相同的限制性图谱。通过对RPV DNA的电泳分析，其大小为118×10⁶道尔顿，比VV DNA小约6×10⁶道尔顿。RPV DNA的两个相对末端限制性片段相互交叉杂交。