Schümperli D, Menna A, Schwendimann F, Wittek R, Wyler R
J Gen Virol. 1980 Apr;47(2):385-98. doi: 10.1099/0022-1317-47-2-385.
Cleavage sites for the restriction endonucleases EcoRI, KpnI and XhoI were mapped on rabbit poxvirus and vaccinia virus DNA. These physical maps were used to analyse the structural variations between the two DNAs. Two specific heterologous regions, symmetrically arranged at each end of the genomes, have been identified. Region 1, representing the exterior part of the terminal repetition, appears to contain unrelated sequences in each DNA and accounts for the difference in length of the two genomes. Region 2, separated from region 1 by a conserved part of the terminal repetition, is located at the transition from repeated to unique DNA sequences. Its overall length of about 4 megadaltons is well conserved and it contains individual DNA-specific as well as conserved restriction sites. The major central part of the genomes (over 100 megadaltons) contains very few, widely dispersed restriction site variations.
限制性内切酶EcoRI、KpnI和XhoI的切割位点已定位在兔痘病毒和痘苗病毒的DNA上。这些物理图谱用于分析两种DNA之间的结构差异。已鉴定出两个特定的异源区域，对称排列在基因组的两端。区域1代表末端重复序列的外部部分，似乎在每种DNA中都包含不相关的序列，并解释了两个基因组长度的差异。区域2与区域1由末端重复序列的保守部分隔开，位于重复DNA序列向单一DNA序列的转变处。其总长度约为4兆道尔顿，保存完好，包含个别DNA特异性以及保守的限制性位点。基因组的主要中心部分（超过100兆道尔顿）包含极少且广泛分散的限制性位点变异。
