Naus C C
Department of Anatomy, University of Western Ontario, London, Canada.
Brain Res Bull. 1990 Apr;24(4):583-92. doi: 10.1016/0361-9230(90)90163-t.
The postnatal appearance of the mRNA for preprosomatostatin, as well as the presence of somatostatin 28 and somatostatin 28, was examined in the rat cerebellum. RNA blotting analysis indicates a dramatic decrease in the level of this mRNA during cerebellar maturation. In situ hybridization reveals that many cells contain this mRNA at early postnatal stages, with progressively fewer cells being labeled after postnatal day 20. Immunohistochemical analysis indicates that there is a changing pattern in the distribution of these somatostatin peptides through cerebellar development. Initially, cells in the granule cell layer are stained, and fiber immunoreactivity is localized primarily in this same layer. During the second postnatal week, this granule cell layer staining decreases, and some Purkinje cells display immunoreactivity, particularly in the flocculus and paraflocculus. This Purkinje cell staining decreases by postnatal day 30, but immunoreactive cells are still present in the granule cell layer. In contrast, in the adult cerebellum, very few of these cells remain immunoreactive.
研究人员在大鼠小脑中检测了前促生长抑素原mRNA的产后表现以及生长抑素28和生长抑素28的存在情况。RNA印迹分析表明，在小脑成熟过程中，这种mRNA的水平急剧下降。原位杂交显示，许多细胞在出生后早期阶段含有这种mRNA，出生后第20天之后被标记的细胞逐渐减少。免疫组织化学分析表明，在小脑发育过程中，这些生长抑素肽的分布模式不断变化。最初，颗粒细胞层中的细胞被染色，纤维免疫反应主要定位于同一层。在出生后的第二周，这种颗粒细胞层染色减少，一些浦肯野细胞显示出免疫反应，特别是在绒球和旁绒球中。这种浦肯野细胞染色在出生后第30天减少，但免疫反应性细胞仍存在于颗粒细胞层中。相比之下，在成年小脑中，这些细胞中很少有保持免疫反应性的。
