Inagaki S, Shiosaka S, Sekitani M, Noguchi K, Shimada S, Takagi H
First Department of Anatomy, Osaka City University Medical School, Japan.
Brain Res Mol Brain Res. 1989 Dec;6(4):289-95. doi: 10.1016/0169-328x(89)90074-0.
We used in situ hybridization histochemistry to examine the postnatal development of the somatostatin (SRIF) synthesizing system in the cerebellum of rats. There are numerous hybridizing neurons from 1 to 9 days after birth. These occur throughout the cerebellum including the developing medulla and cortex except in the external granular cell layer. The lateral cerebellar nucleus also contains SRIF gene-containing cells. The intensity of the signals for SRIF mRNA in the cerebellum decreases with age. There is a drastic decrease in SRIF mRNA in the lateral cerebellar nucleus. SRIF cells cannot be detected in the lateral cerebellar nucleus of adult rats, whereas a small, yet significant number of SRIF cells are scattered in the cerebellar medulla. However, the cerebellum of adult rats still contains a significant number of labeled cells in the granular cell layer, although the intensity for SRIF mRNA decreases from 14 days after birth to adulthood. SRIF gene-expressing cells in the cerebellar cortex are located primarily in the granular cell layer and appear to correspond to Golgi cells judging from their characteristic features. These results are consistent with our previous immunohistochemical study on the decrease of SRIF immunoreactivity in the cerebellum of adult rats. These findings, together with a recent study of transient SRIF receptor-expressing cells in the developing cerebellum suggest that SRIF acts during cerebellar development.
我们运用原位杂交组织化学技术来研究大鼠小脑内生长抑素（SRIF）合成系统的出生后发育情况。出生后1至9天存在大量杂交神经元。这些神经元遍布小脑，包括发育中的髓质和皮质，但外颗粒细胞层除外。小脑外侧核也含有含SRIF基因的细胞。小脑中SRIF mRNA的信号强度随年龄增长而降低。小脑外侧核中SRIF mRNA急剧减少。在成年大鼠的小脑外侧核中检测不到SRIF细胞，而少量但数量可观的SRIF细胞散在于小脑髓质中。然而，成年大鼠的小脑颗粒细胞层中仍含有大量标记细胞，尽管从出生后14天到成年期SRIF mRNA的强度有所下降。小脑皮质中表达SRIF基因的细胞主要位于颗粒细胞层，从其特征判断似乎对应于高尔基细胞。这些结果与我们之前关于成年大鼠小脑SRIF免疫反应性降低的免疫组织化学研究一致。这些发现，连同最近对发育中小脑内短暂表达SRIF受体细胞的研究表明，SRIF在小脑发育过程中发挥作用。
