Cauli B, Audinat E, Lambolez B, Angulo M C, Ropert N, Tsuzuki K, Hestrin S, Rossier J
Neurobiologie et Diversité Cellulaire, Centre National de la Recherche Scientifique Unité de Recherche Associée 2054, Ecole Supérieure de Physique et de Chimie Industrielles de la ville de Paris, 75231 Paris cedex 5, France.
J Neurosci. 1997 May 15;17(10):3894-906. doi: 10.1523/JNEUROSCI.17-10-03894.1997.
The physiological and molecular features of nonpyramidal cells were investigated in acute slices of sensory-motor cortex using whole-cell recordings combined with single-cell RT-PCR to detect simultaneously the mRNAs of three calcium binding proteins (calbindin D28k, parvalbumin, and calretinin) and four neuropeptides (neuropeptide Y, vasoactive intestinal polypeptide, somatostatin, and cholecystokinin). In the 97 neurons analyzed, all expressed mRNAs of at least one calcium binding protein, and the majority (n = 73) contained mRNAs of at least one neuropeptide. Three groups of nonpyramidal cells were defined according to their firing pattern. (1) Fast spiking cells (n = 34) displayed tonic discharges of fast action potentials with no accommodation. They expressed parvalbumin (n = 30) and/or calbindin (n = 19) mRNAs, and half of them also contained transcripts of at least one of the four neuropeptides. (2) Regular spiking nonpyramidal cells (n = 48) displayed a firing behavior characterized by a marked accommodation and presented a large diversity of expression patterns of the seven biochemical markers. (3) Finally, a small population of vertically oriented bipolar cells, termed irregular spiking cells (n = 15), fired bursts of action potentials at an irregular frequency. They consistently co-expressed calretinin and vasoactive intestinal polypeptide. Additional investigations of these cells showed that they also co-expressed glutamic acid decarboxylase and choline acetyl transferase. Our results indicate that neocortical nonpyramidal neurons display a large diversity in their firing properties and biochemical patterns of co-expression and that both characteristics could be correlated to define discrete subpopulations.
利用全细胞膜片钳记录技术结合单细胞逆转录聚合酶链反应，在感觉运动皮层急性脑片中研究了非锥体神经元的生理和分子特征，以同时检测三种钙结合蛋白（钙结合蛋白D28k、小白蛋白和钙视网膜蛋白）和四种神经肽（神经肽Y、血管活性肠肽、生长抑素和胆囊收缩素）的信使核糖核酸。在所分析的97个神经元中，所有神经元均表达至少一种钙结合蛋白的信使核糖核酸，大多数（n = 73）含有至少一种神经肽的信使核糖核酸。根据放电模式定义了三组非锥体神经元。（1）快速放电细胞（n = 34）表现为快速动作电位的强直放电，无适应性变化。它们表达小白蛋白（n = 30）和/或钙结合蛋白（n = 19）的信使核糖核酸，其中一半还含有四种神经肽中至少一种的转录本。（2）规则放电非锥体神经元（n = 48）表现出以明显适应性为特征的放电行为，并呈现出七种生化标志物的多种表达模式。（3）最后，一小群垂直定向的双极细胞，称为不规则放电细胞（n = 15），以不规则频率发放动作电位爆发。它们始终共同表达钙视网膜蛋白和血管活性肠肽。对这些细胞的进一步研究表明，它们还共同表达谷氨酸脱羧酶和胆碱乙酰转移酶。我们的结果表明，新皮层非锥体神经元在放电特性和共表达的生化模式方面表现出很大的多样性，并且这两种特征可能相互关联以定义离散的亚群。
