通过琼脂糖凝胶电泳分析多瘤病毒DNA复制中间体
Analysis of polyoma virus DNA replicative intermediates by agarose gel electrophoresis.
作者信息
Martin R F
出版信息
J Virol. 1977 Sep;23(3):827-32. doi: 10.1128/JVI.23.3.827-832.1977.
Abstract
Agarose gel electrophoresis has been used to fractionate polyoma virus DNA replicative intermediates (RI) according to maturity. Approximate electrophoretic mobility versus maturity relationships were obtained for both intact (supercoiled) and nicked (relaxed) RI. There was considerable overlap between the supercoiled and relaxed RI populations after electrophoretic fractionation. Intact RI could be recovered from preparative agarose gels for further analysis by centrifugation, electron microscopy, re-electrophoresis, or nuclease digestion.
摘要
琼脂糖凝胶电泳已被用于根据成熟度对多瘤病毒DNA复制中间体（RI）进行分级分离。完整（超螺旋）和切口（松弛）的RI均获得了近似的电泳迁移率与成熟度关系。电泳分级分离后，超螺旋和松弛的RI群体之间存在相当大的重叠。完整的RI可以从制备性琼脂糖凝胶中回收，以便通过离心、电子显微镜、重新电泳或核酸酶消化进行进一步分析。
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