用核酸内切酶R-RI将2型腺病毒DNA切割成六个独特的片段。
Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.
作者信息
Pettersson U, Mulder C, Deluis H, Sharp P A
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):200-4. doi: 10.1073/pnas.70.1.200.
Abstract
The DNA of adenovirus type 2 was cleaved by restriction endonuclease R.RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 x 10(6) to 13.6 x 10(6), as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.
摘要
2型腺病毒的DNA被限制性内切酶R.RI切割成六个片段。这些片段通过在复合琼脂糖-聚丙烯酰胺凝胶上进行电泳分离。通过电子显微镜测量，它们的分子量范围从1.1×10⁶到13.6×10⁶。每个片段代表2型腺病毒DNA的一个独特区段，因为：（i）片段以等摩尔量获得；（ii）它们分子量的总和等于完整腺病毒DNA的分子量；并且（iii）每个片段表现出的复性速率与其大小成反比。
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