Aniksztejn L, Charriaut-Marlangue C, Roisin M P, Ben-Ari Y
Laboratoire de Neurobiologie et Physiopathologie du Développement, INSERM U29, Paris, France.
Neuroscience. 1990;35(1):63-70. doi: 10.1016/0306-4522(90)90120-s.
Using a push-pull device, we have analysed, in vivo, the release of endogenous excitatory amino acids and proteins induced by the mast cell degranulating peptide in the CA1 region of the hippocampus. Local application of the mast cell degranulating peptide (20 microM) for 5 or 10 min produced a long-term potentiation of the slope of the field excitatory postsynaptic potential (70 +/- 40%, 3 h after the drug application). This long-term potentiation was associated with (i) a transient increase (10 min) in the release of endogenous glutamate and aspartate and (ii) a late transient enhanced release of proteins and newly secreted proteins. In cases in which the mast cell degranulating peptide induced recurrent interictal activity, there was a sustained enhanced release of glutamate. These observations suggest that mast cell degranulating peptide induced long-term potentiation is not associated with a sustained enhanced release of excitatory amino acids.
我们使用一种推挽式装置，在体分析了海马CA1区肥大细胞脱颗粒肽诱导的内源性兴奋性氨基酸和蛋白质的释放。局部应用肥大细胞脱颗粒肽（20微摩尔）5或10分钟可产生场兴奋性突触后电位斜率的长期增强（给药后3小时，增强70±40%）。这种长期增强与以下情况相关：（i）内源性谷氨酸和天冬氨酸释放的短暂增加（10分钟），以及（ii）蛋白质和新分泌蛋白质的后期短暂增强释放。在肥大细胞脱颗粒肽诱导反复发作间期活动的情况下，谷氨酸释放持续增强。这些观察结果表明，肥大细胞脱颗粒肽诱导的长期增强与兴奋性氨基酸的持续增强释放无关。
