Aniksztejn L, Roisin M P, Gozlan H, Ben-Ari Y
INSERM U29, Hopital de Port-Royal, Paris, France.
Neurosci Lett. 1987 Oct 29;81(3):291-5. doi: 10.1016/0304-3940(87)90398-3.
The relationship between the long-lasting enhancement of synaptic transmission produced by a phorbol ester and the release of endogenous excitatory amino acids has been investigated in the CA1 hippocampal region of the anaesthetized rat. Using the push-pull technique, the concentration of glutamate and aspartate was assayed in the perfusate by high-pressure liquid chromatography. Application of phorbol 12-13 diacetate produced a long lasting enhancement of the field excitatory postsynaptic potential (EPSP) (over 2 h). This was associated with a brief (10 min) significant increase in the release of glutamate and aspartate. However, subsequently the levels of the amino acids in the perfusate were not different from the pre-drug (control) levels although the field EPSP was still enhanced. It is concluded that the long-lasting enhancement produced by phorbol ester is not due to a persistent increase in the release of excitatory amino acids.
在麻醉大鼠的海马CA1区，研究了佛波酯产生的突触传递长期增强与内源性兴奋性氨基酸释放之间的关系。采用推挽技术，通过高压液相色谱法测定灌流液中谷氨酸和天冬氨酸的浓度。应用佛波醇12 - 13二乙酸酯可使场兴奋性突触后电位（EPSP）产生长期增强（超过2小时）。这与谷氨酸和天冬氨酸释放的短暂（10分钟）显著增加有关。然而，随后尽管场EPSP仍增强，但灌流液中氨基酸水平与给药前（对照）水平并无差异。得出的结论是，佛波酯产生的长期增强并非由于兴奋性氨基酸释放的持续增加。
