麻醉大鼠海马体在长时程增强过程中的蛋白质释放

Release of proteins during long-term potentiation in the hippocampus of the anaesthetized rat.

作者信息

Charriaut-Marlangue C, Aniksztejn L, Roisin M P, Ben-Ari Y

机构信息

INSERM U 29, Unité de Neurobiologie et Physiopathologie du developpement, Hôpital de Port-Royal, Paris, France.

出版信息

Neurosci Lett. 1988 Sep 12;91(3):308-14. doi: 10.1016/0304-3940(88)90698-2.

DOI:10.1016/0304-3940(88)90698-2

PMID:2903470

Abstract

Using a push-pull device, the release of endogenous proteins in the extracellular space was investigated in the CA1 region of the hippocampus of anaesthetized rats. With low-frequency stimulation of the Schaffer collaterals, there was a relatively stable release of 5 proteins (64, 54, 48, 45 and 16 kDa). A train of high-frequency stimulation produced a long-lasting enhancement of the negative field EPSP and a delayed (90-120 min) enhancement of the release of these proteins. An additional 19 kDa protein was present only 90 min after the train. These observations raise the possibility that release of proteins might be involved in the maintenance of LTP.

摘要

利用推挽式装置，在麻醉大鼠海马体的CA1区研究细胞外空间内源性蛋白质的释放情况。对谢弗侧支进行低频刺激时，有5种蛋白质（64、54、48、45和16 kDa）呈现相对稳定的释放。一串高频刺激产生了负向场兴奋性突触后电位的持久增强以及这些蛋白质释放的延迟（90 - 120分钟）增强。在高频刺激串后仅90分钟出现了一种额外的19 kDa蛋白质。这些观察结果增加了蛋白质释放可能参与长时程增强维持的可能性。