Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Curr Microbiol. 2010 Jan;60(1):1-5. doi: 10.1007/s00284-009-9490-z. Epub 2009 Aug 29.
The in vitro cytotoxicity of the antimicrobial peptide P40 was investigated. The food grade bacteriocin nisin was also analyzed for comparison. VERO cells were treated with different concentrations (0.02-2.5 microg ml(-1)) of nisin and P40, and cell viability and plasma membrane integrity were checked by MTT, neutral red uptake (NRU), and lactate dehydrogenase (LDH) assays. In MTT and NRU assays the EC(50) to the purified peptide P40 were 0.30 and 0.51 microg ml(-1), while values found to nisin were 0.35 and 0.79 microg ml(-1), respectively. In the LDH assay, the EC(50) was 0.57 and 0.62 microg ml(-1) for P40 and nisin, respectively. The peptide P40 revealed higher hemolytical activity (19%) when compared to nisin (4.9%) at the highest concentration tested (2.5 microg ml(-1)). Relatively few studies about the cytotoxicity of antimicrobial peptides are available. The determination of the cytotoxicity of antimicrobial peptides is an essential step to warrant their safe use.
研究了抗菌肽 P40 的体外细胞毒性。同时也分析了食品级细菌素乳链菌肽作为比较。用不同浓度（0.02-2.5μg/ml）的乳链菌肽和 P40 处理 VERO 细胞，通过 MTT、中性红摄取（NRU）和乳酸脱氢酶（LDH）测定法检查细胞活力和质膜完整性。在 MTT 和 NRU 测定中，纯化肽 P40 的 EC50 分别为 0.30 和 0.51μg/ml，而乳链菌肽的 EC50 分别为 0.35 和 0.79μg/ml。在 LDH 测定中，P40 和乳链菌肽的 EC50 分别为 0.57 和 0.62μg/ml。在测试的最高浓度（2.5μg/ml）下，肽 P40 的溶血活性（19%）高于乳链菌肽（4.9%）。关于抗菌肽细胞毒性的研究相对较少。抗菌肽的细胞毒性测定是保证其安全使用的必要步骤。
