Department of Surgery, SMZ Ost and Cluster Translational Oncology, Ludwig Boltzmann Society, Langobardenstrasse 122, Vienna, Austria.
J Gastrointest Surg. 2009 Nov;13(11):2020-6. doi: 10.1007/s11605-009-0992-6. Epub 2009 Sep 2.
Soluble cytokeratin 18 fragments (M30, M65) are released from human cancer cells during cell death and hold potential as biomarkers in colorectal cancer characterized by frequent metastatic spread. A total of 62 colorectal cancer and 27 control patients were included in the study. M65 (necrosis and apoptosis) and M30 (apoptosis) were quantified preoperatively (n = 62) and postoperatively (n = 31) using specific enzyme-linked immunosorbent assays. Presence of disseminated tumor cells (DTC) in the bone marrow was assessed by staining of A45-B/B3-positive cells in aspirates. M65 was significantly elevated in patients with International Union against Cancer stage I and IIA tumors compared to controls. A subgroup (19/31) exhibited a significant (p < 0.05) decrease of M65 after tumor surgery (503.9 +/- 230.7 to 342.6 + 94.8 U/l; -32.0 +/- 16.5%), in contrast to 12 patients who revealed higher M65 levels postoperatively (386.5 +/- 128.5 to 519.1 +/- 151 U/l; +37.4 +/- 32.3%). DTC in bone marrow were found in 10% (2/19) of patients with decreasing and 50% (6/12) of the patients with increasing M65 serum concentrations after surgery (p = 0.028). In conclusion, M65 as marker is likely to be valuable to identify patients with a high incidence of systemic disease.
可溶性细胞角蛋白 18 片段（M30、M65）在细胞死亡时从人类癌细胞中释放出来，并有可能成为经常发生转移扩散的结直肠癌的生物标志物。共有 62 名结直肠癌患者和 27 名对照患者纳入本研究。使用特异性酶联免疫吸附试验分别在术前（n=62）和术后（n=31）检测 M65（坏死和凋亡）和 M30（凋亡）。通过染色 A45-B/B3 阳性细胞评估骨髓中播散性肿瘤细胞（DTC）的存在。与对照组相比，国际抗癌联盟（UICC）I 期和 IIA 期肿瘤患者的 M65 显著升高。亚组（19/31）在肿瘤手术后 M65 显著降低（503.9+/-230.7 至 342.6+/-94.8 U/l；-32.0+/-16.5%），而 12 名患者术后 M65 水平升高（386.5+/-128.5 至 519.1+/-151 U/l；+37.4+/-32.3%）。术后 M65 血清浓度降低的 19 名患者中有 2 名（10%）和 M65 血清浓度升高的 12 名患者中有 6 名（50%）骨髓中发现 DTC（p=0.028）。总之，M65 作为标志物可能有助于识别系统疾病发生率高的患者。
