Van Camp G, Van Hul W, Backhovens H, Stinissen P, Wehnert A, Patterson D, Vandenberghe A, Van Broeckhoven C
Department of Biochemistry, University of Antwerp, Belgium.
Somat Cell Mol Genet. 1990 May;16(3):241-9. doi: 10.1007/BF01233360.
From a chromosome 21 phage library, we selected 10 clones located proximal of the senile plaque amyloid precursor protein gene. Since a locus for Alzheimer's disease (AD) has been localized in the pericentromeric region of chromosome 21, the selected phage clones are potential candidate probes for genetic analysis of AD. In this study, we subcloned single-copy fragments of the selected phage clones, refined their physical localization, and examined their chromosomal distribution in relation to their position on chromosome 21. The results indicated that the phage clones are identifying nine chromosome 21 loci, which, if polymorphic, may be helpful in localizing the AD locus more precisely. Moreover, since all phage clones are located close to the centromere of chromosome 21, they can be used to determine the parental origin of nondisjunction in trisomy 21 with high reliability.
从一个21号染色体噬菌体文库中，我们挑选了10个位于老年斑淀粉样前体蛋白基因近端的克隆。由于阿尔茨海默病（AD）的一个基因座已定位在21号染色体的着丝粒周围区域，因此所选的噬菌体克隆是AD遗传分析的潜在候选探针。在本研究中，我们对所选噬菌体克隆的单拷贝片段进行亚克隆，完善其物理定位，并研究它们在21号染色体上的位置与其染色体分布的关系。结果表明，这些噬菌体克隆鉴定出了9个21号染色体基因座，如果这些基因座具有多态性，可能有助于更精确地定位AD基因座。此外，由于所有噬菌体克隆都位于21号染色体着丝粒附近，它们可用于高可靠性地确定21三体中不分离的亲本来源。
