Patho-TB 试剂盒与直接显微镜检查和培养比较，快速检测痰中的结核分枝杆菌。

Rapid detection of Mycobacterium tuberculosis in sputum by Patho-TB kit in comparison with direct microscopy and culture.

机构信息

Microbiology and Immunology Laboratory UR02SP13, CHU Farhat Hached, Sousse 4000, Tunisia.

出版信息

Diagn Microbiol Infect Dis. 2009 Nov;65(3):232-5. doi: 10.1016/j.diagmicrobio.2009.07.021. Epub 2009 Sep 2.

DOI:10.1016/j.diagmicrobio.2009.07.021

PMID:19729264

Abstract

The usefulness of a new rapid diagnostic test (Patho-TB) using antibodies specific to mycobacterial antigens was evaluated for the rapid discrimination between pulmonary tuberculosis (TB) and non-TB pulmonary diseases on sputa. One hundred sputa collected from 79 active TB patients and from 21 patients with non-TB pulmonary diseases (asthma and chronic obstructive pulmonary disease) were enrolled into the study and tested for the presence of Mycobacterium tuberculosis by Ziehl-Neelsen smear, Patho-TB kit, and Löwenstein-Jensen culture. The sensitivity, specificity, positive predictive value, and negative predictive value of the Patho-TB test were 95%, 100%, 100%, and 84%, respectively. Patho-TB test is simple, quick, and easy to perform. Its sensitivity, specificity, and positive predictive value are satisfactory. Therefore, it could be used as a screening test in poorly equipped laboratories of TB endemic areas.

摘要

本研究旨在评估一种新的快速诊断检测方法（Patho-TB）在痰液中用于快速区分肺结核（TB）和非 TB 肺部疾病的效果。共收集了 79 例活动性肺结核患者和 21 例非 TB 肺部疾病（哮喘和慢性阻塞性肺疾病）患者的 100 份痰液，分别通过 Ziehl-Neelsen 染色、Patho-TB 试剂盒和 Löwenstein-Jensen 培养法检测结核分枝杆菌的存在。Patho-TB 检测的敏感性、特异性、阳性预测值和阴性预测值分别为 95%、100%、100%和 84%。Patho-TB 检测简单、快速、易于操作。其敏感性、特异性和阳性预测值均令人满意。因此，它可作为结核流行地区设备较差的实验室的筛查检测方法。